a83 01 (Tocris)

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Tocris a83 01
A83 01, supplied by Tocris, used in various techniques. Bioz Stars score: 98/100, based on 1957 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/a83+01/A+83-01/pmc13091043-18-0-2
Average 98 stars, based on 1957 article reviews

a83 01 - by Bioz Stars, 2026-10

98/100 stars

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Produced:

Article Title: Asymmetric crosstalk between the BMP and TGFβ pathways resolves signaling ambiguity
Article Snippet: 36 hours following media replacement, cells were then imaged using the CellDiscoverer 7 microscope (Zeiss). .. Patient-derived tumor breast organoids were overlaid with organoid culture medium containing Advanced DMEM DMEM/F12 (Thermofisher; 12634010), supplemented with 10mM HEPES (GIBCOTM; 15630-080), 2mM GlutaMAX (GIBCOTM; 35050-061), x1 Penicillin streptomycin (Biowest; L0022-100), 10% R-spondin-1-conditioned medium (RCM) produced from HEK293 HA–Rspo1–Fc cells (Cultrex® HA–R-spondin-1–Fc 293T cells; 3710-001-01), 10% Noggin-conditioned medium (NCM) produced from HEK293 cells stably transfected with pcDNA3-mouse NEO insert (to confer neomycin resistance; cells for NCM production were kindly provided by the Hubrecht Institute, Utrecht, The Netherlands), x1 B27 (ThermoFisher; 17504044)), 100 ng/ml A83-01 (Tocris Bioscience; 2939), 50 ng/ml EGF (PeproTech; AF-100-15). .. When organoids were first established or expanded, 10 mM Y-27632 (ROCK inhibitor; Sigma Aldrich; Y0503) was added to the medium.

Stable Transfection:

Transfection:

Generated:

Article Title: Hdac11 promotes idiopathic pulmonary fibrosis through macrophage M2-type polarization and myofibroblast accumulation by inhibiting Parkin-dependent mitophagy.
Article Snippet: .. Then, lung bud-like progenitor clusters were generated by sequential treatment with anteriorized medium for 3 d and with ventralized medium for another 3 d. Anteriorized medium was comprised of 100 ng/mL NOG (#6057-NG, R&D Systems, USA) and 5 μM A83-01 (#2939/10, Tocris, UK), while ventralized medium contained 3 μM CHIR99021 (#4423/10, Tocris, UK), 10 ng/mL BMP4, 10 ng/mL FGF7 (#251-KG, R&D Systems, USA), 10 ng/mL FGF10 (#345-FG, R&D systems, USA), 50 nM RA (#0695/50, Tocris, UK), 10 ng/mL VEGF (#298-VS, R&D Systems, USA) and 10 ng/mL SCF (#11010-SC, R&D Systems, USA). ..

Cell Culture:

Article Title: Characterization and therapeutic suppression of KEAP1-NRF2-driven resistance to KRAS inhibitors in pancreatic and lung cancer
Article Snippet: .. The organoids were cultured in reduced growth factor Matrigel domes with compete human feeding medium (Advanced DMEM/F12 (Thermo Fisher, #12634028)–based WRN conditioned medium (L-WRN)(ATCC, CRL-3276), 100 ng/mL hFGF10 (Thermo Fisher # 100-26), 10 mM nicotinamide (Sigma #72340), 0.01 μM hGastrin I (TOCRIS #3006), 50 ng/mL hEGF (Thermo Fisher # AF-100-15), 500 nM A83-01 (TOCRIS #2939), 1× B27 supplement (Thermo Fisher, #A1486701), 10 mM HEPES (Thermo Fisher #15630130), and 0.01 μM GlutaMAX (all Thermo Fisher, #35050079). ..

Recombinant:

Article Title: Application of a High-Biomimetic Tumor Organoid-CAF Co-Culture Model for the Efficacy Evaluation of CAR-T Drugs
Article Snippet: .. It is supplemented with a sophisticated blend of active ingredients, which includes: 1X B27, Glutamax, 10 mM HEPES (15630080, Gibco), 100 μg/mL primocin (ant-pm-2, Invivo-Gen), 50 ng/mL recombinant human EGF (AF-100-15, Peprotech), 10 nM gastrin (G9020, Sigma), 500 nM A83-01 (2939, Tocris Bioscience), 1.25 mM N - acetylcysteine (A9165, Sigma), 10 mM nicotinamide (N0636, Sigma), 100 ng/mL recombinant human Noggin (120-10C, Peprotech), and 20% R - spondin1 conditioned media. ..

Review